Table 1.
Quantitative analysis of the distribution and synaptic localisation of GABABR subunit puncta in neurons at 14 and 21 DIV. Regions of dendrite were selected at random and the immunoreactive puncta counted. A puncta was defined as a spot of immunofluorescence showing at least twice the background level of fluorescence. Puncta was classed as co-localised if greater than 50% of the pixels were overlapping. The data are the mean and SEM taken from at least three different fields in three separate cell culture preparations
| Puncta per 100 μm | Puncta co-localised with SV2a per 100 μm |
Percentage of GABABR subunit puncta co-localised with SV2a |
Percentage of SV2a puncta co-localised with GABABR subunit |
|
|---|---|---|---|---|
| 14 DIV | ||||
| GABAB1 | 30 ± 1.7 | 19 ± 0.6 | 56 ± 1.1 | – |
| SV2a | 38 ± 3.5 | – | – | 49 ± 5.2 |
| GABAB2 | 29 ± 1.1 | 14 ± 0.6 | 49 ± 4.1 | – |
| SV2a | 38 ± 2.3 | – | – | 50 ± 2.3 |
| 21 DIV | ||||
| GABAB1 | 36 ± 2.9 | 18 ± 0.6 | 67 ± 4.6 | – |
| SV2a | 56 ± 6.3 | – | – | 45 ± 1.1 |
| GABAB2 | 39 ± 3.5 | 25 ± 2.3 | 71 ± 1.7 | – |
| SV2a | 63 ± 2.3 | – | – | 42 ± 1.1 |