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. Author manuscript; available in PMC: 2013 Mar 6.
Published in final edited form as: Oncogene. 2011 Dec 19;31(36):4076–4084. doi: 10.1038/onc.2011.585

Fig 4.

Fig 4

Chromatin bound MCM2 expression and phosphorylation is affected by hypoxic activation of ATR. A. MCM2 expression in hypoxic (Hy) and normoxic (N) cells was assessed in cytoplasmic, chromatin bound, and nucleoplasm cell compartments. Chromatin bound MCM2 was also treated with nuclease to confirm proper fractionation. B. Soluble and chromatin bound MCM2 in normoxic and hypoxic U2OS control and ATR depleted cells (note, panel is a composite). Proteins were resolved so that two bands were apparent, the bottom one consistent with a phosphorylated form, as C. demonstrated by its disappearance with lambda phosphatase treatment. D. Soluble and Chromatin bound phosphorylated forms of MCMC2 in normoxic and six hours hypoxic U2OS control and ATR depleted cells.