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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Oncogene. 2011 Dec 19;31(39):4317–4332. doi: 10.1038/onc.2011.593

Figure 3. PIT-1 suppresses ARF6 but not ARF6-Q67L induced lamellipodia formation.

Figure 3

(A,B) PITs inhibit lamellipodia formation induced by ARF6 overexpression. SUM159 cells were transfected with ARF6 or ARAP3, or co-transfected with both. Then cells were serum starved and treated with compounds for 2 hr, followed by stimulation with 100 ng/ml PDGF for 15 min. Cells were stained and analyzed with fluorescent microscopy. The representative fluorescent images are shown (A, the lamellipodia are indicated by arrowheads). The number of cells with significant lamellipodia was counted randomly in five fields and the percentage was calculated (B)

(C,D) PITs fail to inhibit lamellipodia formation induced by ARF6-Q67L overexpression. SUM159 cells were transfected with ARF6-Q67L. Then cells were treated with compounds for 2 hr, followed by staining and fluorescent microscopy analysis. The representative fluorescent images are shown (C) and the number of cells with significant lamellipodia was counted in five random fields and the percentage was calculated (D).

##P<0.01 compared with empty vector group, **P<0.01 compared with ARF6 overexpression control groups.