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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Oncogene. 2011 Dec 19;31(39):4317–4332. doi: 10.1038/onc.2011.593

Figure 6. PIT-1 suppresses ARF6 or GRP1/ARNO induced cell migration.

Figure 6

(A,B) PITs inhibit ARF6-induced cell migration. SUM159 cells were transfected with ARF6. Scratch wound was generated in cell monolayer, followed by treatment with PITs (50 μM) for 8 hr. The width of wounded cell monolayers was measured in five random fields and expressed as % of original width (A). The wound width ratio (PITs treated groups/DMSO control group) was calculated (B).

(C) Overexpression of ARF6-Q67L attenuates inhibition of cell migration led by PIT-1. SUM159 cells were transfected with ARF6-Q67L. Scratch wound was generated in cell monolayer, followed by treatment with compounds for 8 hr. The width of wounded cell monolayer was measured in five random fields, and the inhibition was calculated.

(D,E) PITs inhibit GRP1/ARNO-induced cell migration. SUM159 cells were transfected with full length GRP1 or ARNO. Scratch wound was generated in cell monolayer, followed by treatment with PITs (50 μM) for 8 hr. The width of wounded cell monolayers was measured in five random fields and expressed as % of original width (D). The wound width ratio (PITs treated groups/DMSO control group) was calculated (E).

(F) Overexpression of EFA6 attenuates inhibition of cell migration by PIT-1. SUM159 cells were transfected with EFA6. Scratch wound was generated in cell monolayer, followed by treatment with compounds for 8 hr. The width of wounded cell monolayer was measured in five random fields, and the inhibition was calculated.

*P<0.05, **P<0.01 compared with empty vector group.