Fig. 1.

a Superposition of yeast tRNA^Phe structure—grey (PDB: 1TRA) and tRNA^Phe with Pb²⁺—black (PDB: 1TN2), in separate box is shown the details of reaction site structure; b HHP effect on Pb²⁺ induced yeast tRNA^Phe hydrolysis. The autoradiogram of the 10% PAGE with 7 M urea of the cleavage reaction of 6.7 μM (300,000 cpm) [3′-³²P]tRNA^Phe induced with 0.5 mM Pb²⁺ in 10 mM Tris–HCl pH 6.8, 10 mM MgCl₂ and 0.4 M NaCl at 22°C, L alkaline ladder; T1 limited hydrolysis with RNase T1; K control without Pb²⁺; K _ap hydrolysis with Pb²⁺ at AP for 120 min; at 400, 600, 800 and 200 MPa, 1–4 respectively at 1, 2, 4 and 16 h. Arrows depict location of the cleavage product on the gel, ap ambient pressure (0.1 MPa)