Figure 2.

Examination on fukutin-over-expressed astrocytoma cells. (a) Morphology of astrocytoma cells after transfection of fukutin. Cells became epithelioid after transfection of fukutin. (b) Confirmation of transfection by PCR and western blotting. PCR using primers of fukutin and vector shows a band, and western blotting of fukutin revealed a stronger reaction as a band at about 60 kDa. There are no bands in a negative control. (c) On examination of laminin-binding ability, fukutin-over-expressed cells attached more to the laminin-coated dish than in the control. FKTN-exp, fukutin-over-expressed cells; T7 and BGH, elements of vector; NC, negative control.