Figure 5.

RNA immunoprecipitation and ELISA-based RNA-binding assay for Musashi-1. (a) RT-PCR in materials after RNA immunoprecipitation using two types of anti-Musashi-1 antibodies. In RT-PCR for fukutin, stronger reaction is seen in materials using anti-Musashi-1 antibodies than in the negative control. No bands are seen in RT-PCR for POMGnT1. In western blotting, a band at about 39 kDa is seen in a material immunoprecipitated with Musashi-1, but a weak band is also observed in a reaction without primary antibody. (b) ELISA-based RNA-binding assay showed an increase of absorption only in a solution containing RNA, extract, probe and anti-digoxigenin antibody. Msi-1, Musashi-1; FKTN, fukutin; POMGnT1, protein O-linked mannose β1,2-N-acetylglucosaminyltransferase; NC, negative control; IP, immunoprecipitation; WB, western blotting.