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RNA immunoprecipitation and ELISA-based RNA-binding assay for vimentin. (a) RNA immunoprecipitation using anti-vimentin antibody. In RT-PCR for fukutin, a band is observed only in a material using anti-vimentin antibody. Western blotting of vimentin reveals a band at about 57 kDa only in a material using anti-vimentin antibody. (b) ELISA-based RNA-binding assay shows no apparent increase of absorption. FKTN, fukutin; NC, negative control; IP, immunoprecipitation; WB, western blotting.
