Figure 1.

Identification of native MT1721 antigen in the culture filtrate of Mycobacterium tuberculosis. Purified rMT1721 was electrophoresed under reducing conditions in 4–20% gradient SDS–PAGE and stained with Coomassie Blue (a). In parallel, rMT1721 and culture filtrate samples were run in SDS–PAGE under similar conditions and transferred to a PVDF membrane followed by probing with rabbit anti-rMT1721 antiserum. Reactivity was detected with horseradish peroxidase-labelled goat anti-rabbit IgG and developed using the enhanced chemiluminescent reagent (b). Lane 1, culture filtrate; lane 2, purified rMT1721.