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. 2012 Mar;135(3):216–225. doi: 10.1111/j.1365-2567.2011.03525.x

Figure 4.

Figure 4

Heterologous DNA and recombinant protein prime/boost immunization induces robust Mycobacterium tuberculosis rMT1721-specific CD4+ cells producing interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α) and interleukin-2 (IL-2). Splenocytes from three mice per group were isolated 12 days after immunization. The splenocytes were exposed to rMT1721 and cytokine production was measured by monoclonal antibody staining and flow cytometric analysis. (a) Flow cytometer plot of results obtained from one representative individual mouse from each group. (b), Average (± SEM) of the percentage of IFN-γ-producing, TNF-α-producing and IL-2-producing CD4+ T cells obtained from three mice per group following stimulation with rMT1721. Mice receiving the DNA-MT1721 prime/rMT1721 boost generated markedly higher CD4 T-cell responses for all three cytokines (IFN-γ, P < 0.021; TNF-α, P < 0.015; and IL-2, P < 0.026) compared with rMT1721 prime/DNA-MT1721 boost.