Table 2. Amino acid sequences of the 34 SIV/HIV peptides used to develop the xMAP gp41 and V3-loop multiplex Luminex assay.
| Peptide | SIV lineage | gp41 peptide sequences | V3-loop peptide sequences |
|---|---|---|---|
| HIV-1 M | HIV1/SIVcpz/gor | LAVERYLKDQQLLGIWGCSGKLIC | NNTRKSVRIGPGQAFYATGDIIGDIRQAYC |
| HIV-1 O | HIV1/SIVcpz/gor | LALGTLIQNQQLLNLWGCKGKLIC | NLTVQEIKIGPMAWYSMGLAAGNGSRAYC |
| HIV-1 N | HIV1/SIVcpz/gor | LAIGRYLRDQQILSLWGCSGKTIC | NNTGGQVGIGPAMTFYNIGKIVGDIRKAYC |
| SIVcpzPts | HIV1/SIVcpz/gor | LAVEKYLRDQQLLSLWGCADKVTC | NRTVRNLQIGPGMTFYNVEIATGDTRKAFC |
| SIVcpzPtt | HIV1/SIVcpz/gor | LAVERYLQDQQILGLWGCSGKAVC | NNTRGEVQIGPGMTFYNIENVVGDTRSAYC |
| SIVgor | HIV1/SIVcpz/gor | LAIETYLRDQQLLGLWGCTGKLIC | NNTRGQIQIGPMTIYNSERIIGNTRKAYC |
| HIV-2/SIVsmm | HIV-2/SIVsmm | TAIEKYLKDQAQLNSWGCAFRQVC | GNKTVVPITLMSGLVFMSQPINKRPRQAWC |
| SIVrcm | SIVrcm | TAIEKYLADQSLLNTFGCAWRQVC | SNRTVKGISLAIGVFISLRVEKRPKGAWC |
| SIVagm | SIVagm | TALEKYLEDQARLNAWGCAWKQVC | GNKTVLPVTIMAGLVFHSQKYNTLLRQAWC |
| SIVgsn | SIVgsn/mus/mon† | SSLEKYLRDQTILQAWGCANRPIC | GNKTIRNLQIGAGMTFYSQVIVGGNTRKAYC |
| SIVmus | SIVgsn/mus/mon† | TALEKFVKDQAILNLWGCANRQIC | † |
| SIVmon | SIVgsn/mus/mon† | TAVEKFIKDQTLLNAWGCANKAVC | † |
| SIVdeb | SIVdeb | TAIEKYLKDQAKLNEWGCAFKQIC | GNKTYRAVHMATGLSFYTTFIPRLRIKRAHC |
| SIVtal | SIVtal‡ | TALEKYLEDQAKLNSWGCAWKQIC | RTIKDLQIAAGLMFHSQIIAGKDLKRAY |
| SIVsyk | SIVsyk | TALETYLRDQAIMSNWGCAFKQIC | GNESIKNIQLAAGYFLPVIQGKLKTGRDAKRAFC |
| SIVlho | SIVlho/sun | TAIEEYLKDQALLASWGCQWKQVC | GNRSEVSTISSTGLLFYYGLEHGSRLRLAQC |
| SIVmnd-2 | SIVmnd | TALEDYVADQSRLAVWGCSFSQVC | GNRSVVSTPSATGLLFYHLGPGKNLKKGMC |
| SIVwrc | SIVwrc | SAIEGFLEDQLKLKQWGCELTQVC | GNRSVVSVNSASGLIYYAGLEPHRNIRKGLC |
| SIVcol | SIVcol‡ | ATIEGYLEEQAKLASIGCANMQIC | GNSSHRNLNTANGAKFYYELIPYSKGIYGRC |
*SIV, simian immunodeficiency virus. †V3 amino acid sequences of SIVgsn, SIVmus, and SIVmon were identical, and only the SIVgsn above the V3-loop peptide was synthesized. ‡Synthesis of SIVcol gp41 and SIVtal V3-loop peptides was unsuccessful.