Table 3. Primers used to amplify simian immunodeficiency virus from dried blood spot samples.
| Primer | Sequences, 5′ → 3′* | Region targeted | Estimated amplicon size, bp | References |
|---|---|---|---|---|
| DR1 | TRCAYACAGGRGCWGAYGA |
pol
|
800 | (8,22) |
| DR2 | AIADRTCATCCATRTAYTG | |||
| DR4 | GGIATWCCICAYCCDGCAGG | 200 |
||
| DR5 |
GGIGAYCCYTTCCAYCCYTGHGG |
|||
| polis4† | CCAGCNCACAAAGGNATAGGAGG |
pol
|
800 | (8,22) |
| polOR† | ACBACYGCNCCTTCHCCTTTC | |||
| polis2† | TGGCARATRGAYTGYACNCAYNTRGAA | 400 |
||
| uni2† |
CCCCTATTCCTCCCCTTCTTTTAAAA |
|||
| polis4† | CCAGCNCACAAAGGNATAGGAGG |
pol
|
800 | (8,22) |
| polOR† | ACBACYGCNCCTTCHCCTTTC | |||
| polis4† | CCAGCNCACAAAGGNATAGGAGG | 650 |
||
| uni2† |
CCCCTATTCCTCCCCTTCTTTTAAAA |
|||
| CNMF1 | TATCCYTCCYTGTCATCYCTCTTT |
pol
|
2,750 | (23) |
| POLor2 | ACBACWGCTCCTTCWCCTTTCCA | |||
| CNMF2 | AATGGAGAATGYTMATAGATTTCAG | 2,050 |
||
| CNMR |
CCCCYATTCCTCCCTTTTTTTTA |
|||
| SPBS | GGCGCCCGAACAGGGACTTG |
gag-pol
|
2,500 | (23) |
| 2500P1 | CCTCCTATGTTCCCCTATTTCTCTG | |||
| CNM.G1 | CGAGGCACTCGGCGATGCTGA | 2,200 |
||
| 2500P2 |
GGAACTGAGAAGGCTGTGTAAGGC |
|||
| 2500L1 | CTATCCCCAAACGCATCCGC |
env-gag
|
2,000 | (23) |
| CNM.G1rev | TCAGCATCGCCGAGTGCCTCG | |||
| 2500L2 | AGAAAAGGGAGGACTGGAAGGGAT | 800 |
||
| SPBSrev |
CAAGTCCCTGTTCGGGCGCC |
|||
| CNMenvF1 | TGTGTSAAAYTRACHCCNATGTGTGT |
env
|
2,480 | (23) |
| CNMenvR1 | AACATNNCYTCYAGTCCTCYCTTTTYT | |||
| CNMenvF2 | TCCTTYAAYCAGACYACAGARTTYAGRGA | 2,140 |
||
| CNMenvR1 |
GGGATAGCCANGAATTNTCNCCAT |
|||
| wrcpolF1 | TAGGGACAGAAAGTATAGTAATHTGG |
pol
|
1,100 | (24) |
| wrcpolR1 | GCCATWGCYAA TGCTGTTTC | |||
| wrcpolF2 | AGAGACAGTAAGGAAGGGAAAGCAGG | 650 |
||
| wrcpolR2 |
GTTCWATTCCTAACCACCAGCADA |
|||
| wrcenvF1 | TGGC AGTGGGACAAAAATATAAAC | env | 750 | (24) |
| wrcenvR1 | CTGGCAGTCCCTCTTCCA AGTT GT | |||
| wrcenvF2 | TGATAGGGMTGGCTCCTGGTGATG | 550 | ||
| wrcenvR2 | AATCCCCATTTYAACCAGTTCCA |
*R = A or G; M = A or C; W = A or T; S = G or C; Y = C or T; B = C, G, or T; H = A, C, or T; and N = A, C, G, or T. †Primers that amplifie simian immunodeficiency virus from dried blood spot samples in this study.