Table 4. Sensitivity and specificity of SIV/HIV peptides used in the xMAP assay to detect SIV infection in human and nonhuman primate samples*.
| Species (common name) | Peptide | Homologous detection |
Homologous and heterologous detection |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SIV+ |
SIV– |
SIV+ |
SIV– |
|||||||||||
| gp41 | V3 | gp41 + V3 | gp41 | V3 | gp41 + V3 | gp41 | V3 | gp41 | V3 | |||||
| Pan troglodytes troglodytes (west central chimpanzee) | SIVcpzPtt | 1/2 | 1/2 | 1/2 | 0/1 | 0/1 | 0/1 | 3/49 | 4/49 | 0/93 | 0/93 | |||
| Pan troglodytes schweinfurthii (eastern chimpanzee) | SIVcpzPts | 1/1 | 1/1 | −/− | −/− | −/− | −/− | 8/49 | 13/49 | 0/93 | 0/93 | |||
| Gorilla gorilla gorilla (western lowland gorilla) | SIVgor | −/− | −/− | −/− | −/− | −/− | −/− | 1/49 | 0/49 | 0/93 | 0/93 | |||
| Cercopithecus nictitans (greater spot-nosed monkey) | SIVgsn | 5/6 | 6/6 | 6/6 | 0/45 | 0/45 | 0/45 | 14/49 | 15/49 | 0/93 | 0/93 | |||
| Cercopithecus cephus (mustached monkey) | SIVmus | 7/7 | 7/7 | 7/7 | 0/30 | 0/30 | 0/30 | 23/49 | 15/49 | 0/93 | 0/93 | |||
| Cercopithecus mona (Mona monkey) | SIVmon | −/− | −/− | −/− | 0/1 | 0/1 | 0/1 | 12/49 | 15/49 | 0/93 | 0/93 | |||
| Miopithecus ogouensis (northern talapoin) | SIVtal | 1/1 | −/− | −/− | 0/6 | 0/6 | 0/6 | 6/49 | −/− | 0/93 | 0/93 | |||
| Cercopithecus neglectus (De Brazza monkey) | SIVdeb | 7/7 | 6/7 | 7/7 | 0/1 | 0/1 | 0/1 | 19/49 | 6/49 | 0/93 | 0/93 | |||
| Cercopithecus albogularis (Sykes’ monkey) | SIVsyk | −/− | −/− | −/− | −/− | −/− | −/− | 11/49 | 1/49 | 0/93 | 0/93 | |||
| Cercocebus atys (sooty mangabey) | SIVsmm | −/− | −/− | −/− | −/− | −/− | −/− | 30/49 | 1/49 | 0/93 | 0/93 | |||
| Cercocebus torquatus (red-capped mangabey) | SIVrcm | 4/4 | 4/4 | 4/4 | −/− | −/− | −/− | 7/49 | 4/49 | 0/93 | 0/93 | |||
| Chlorocebus tantalus (African green monkey) | SIVagm | 1/1 | 1/1 | 1/1 | −/− | −/− | −/− | 5/49 | 3/49 | 0/93 | 2/93 | |||
| Mandrillus sphinx (mandrill) | SIVmnd-2 | 7/10 | 10/10 | 10/10 | 0/2 | 0/2 | 0/2 | 13/49 | 11/49 | 0/93 | 0/93 | |||
| Cercopithecus lhoesti (L'Hoest's monkey) | SIVlho | −/− | −/− | −/− | −/− | −/− | −/− | 6/49 | 6/49 | 0/93 | 0/93 | |||
| Procolobus badius (western red colobus) | SIVwrc | −/− | −/− | −/− | −/− | −/− | −/− | 1/49 | 6/49 | 0/93 | 0/93 | |||
| Colobus guereza (mantled guereza) | SIVcol | −/− | 9/10 | 9/10 | −/− | 0/7 | 0/7 | −/− | 9/49 | 0/93 | 0/93 | |||
| Homo sapiens (human) | HIV-1M | −/− | −/− | −/− | −/− | −/− | −/− | 4/49 | 1/49 | 0/93 | 0/93 | |||
| HIV-1-O | −/− | −/− | −/− | −/− | −/− | −/− | 1/49 | 0/49 | 0/93 | 0/93 | ||||
| HIV-1N | −/− | −/− | −/− | −/− | −/− | −/− | 9/49 | 1/49 | 0/93 | 0/93 | ||||
| Total | 34/39 | 46/49 | 47/49 | 0/87 | 0/87 | 0/87 | 49/49 | 47/49 | 0/93 | 2//93 | ||||
| Sensitivity | 87.2% | 93.9% | 95.9% | NA | NA | NA | 100% | 95.5% | NA | NA | ||||
| Specificity | NA | NA | NA | 100% | 100% | 100% | NA | NA | 100% | 97.9% | ||||
*SIV, simian immunodeficiency virus; −/−, not calculated because homologous serum and/or peptide not available; NA, not applicable.