Figure 6.

Ectopic expression of IRF-8 extinguishes neutrophil potential and induces DC potential in LMPPs, common myeloid progenitors, and GMPs. LMPPs, common myeloid progenitors (CMPs), or GMPs were double-sorted from the BM of wild-type mice. One thousand cells per well were cultured in the presence of Flt3 ligand, GM-CSF, IL-3, and stem cell factor (all 10 ng/mL) for 7 days, and transduced by retrovirus as described in “In vitro culture and retroviral transductions.” Cells were harvested and analyzed for neutrophil (MHC class II⁻GR-1⁺) and DC (MHC class II⁺Gr-1⁻) production in virus-infected (GFP⁺) cells. Representative FACS plots showing the development of GFP⁺ MHC class II⁻ Gr-1⁺ neutrophils and GFP⁺ MHC class II⁺ Gr-1⁻ DCs in (A) LMPPs and common myeloid progenitors or (B) GMPs. Data represent 4 independent experiments with progenitors analyzed in triplicate. (C) GFP⁺ cells were sorted from GMP cultures and morphology of cells was analyzed by Giemsa stain and microscopy.