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. Author manuscript; available in PMC: 2012 Mar 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2010 Jul 26;49(32):5489–5493. doi: 10.1002/anie.201001151

Figure 3. Tertiary structure of ester insulin and its relation to mature insulin, by 2D 1H-NMR.

Figure 3

(Panels a and b) Spectra of ester insulin. Aromatic TOCSY spin systems are shown in panel a). Assignments of Tyr and Phe spin systems are as labeled; NOEs between aormatic protons (vertical axis) and aliphatic protons (horizontal axis) are shown in panel b). (Panels c and d) Corresponding spectra of KP-insulin. Aromatic TOCSY spin systems are shown in panel c) with related NOEs in panel d). Assignment of NOE cross-peaks a-j (a‘-j’) are reported in the Supporting Information. Samples were dissolved in 10 mM deuterioacetic acid (pH 3.0). Spectra were acquired at 25°C and 700 MHz.