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. Author manuscript; available in PMC: 2012 Mar 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2010 Jul 26;49(32):5489–5493. doi: 10.1002/anie.201001151

Figure 4. Conversion of ester insulin to native KP-insulin.

Figure 4

a) saponification of ester insulin 2 to give KP-insulin 3. Conditions were 2: 0.12 mg mL−1, NaOH: 25 mM, acetonitrile-H2O (2.5:7.5), 4 °C. Reaction mixture at T = 2 h (upper panel) and T = 24 h (lower panel), #unrelated column contaminant, *derived from A-chain, **B-chain; b) purified KP-insulin 3. (Inset) On-line ESI-MS spectra taken at the top of the main peak. Chromatographic separations were performed as described in Figure 2 legend; c) binding affinities of synthetic KP-insulin 3 and authentic KP-insulin (purchased from Eli Lilly and Co) to the insulin receptor.