Fig. 7.

CYP2E1KO mice are protected against HFD-induced steatosis. Four-week-old WT and CYP2E1KO mice were maintained on HFD for 12 wk. A: liver sections were prepared and stained with Oil Red O for neutral lipids. B and C: liver triglyceride levels and fatty acid synthase (FAS) protein levels, respectively, were determined as described under experimental procedures. Data represent means ± SE from 4–5 individual mice per group. *P < 0.05. C, inset: 3 representative FAS- and tubulin-loading control immunoblots from WT and CYP2E1KO mouse livers.