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. 2012 Jan 10;302(6):E714–E723. doi: 10.1152/ajpendo.00486.2011

Fig. 6.

Fig. 6.

Partial knock down of Ubc-9 rescues SUMO-mediated impaired trafficking of GLP-1R. MIN6 cells transfected with GLP-1R-GFP and mCherry SUMO-1 were transduced with retroviral particles expressing scrambled or short-hairpin RNA (shRNA) against Ubc-9. A: MIN6 cells expressing GLP-1R-GFP and mCherry SUMO-1 transduced with shRNA scramble showing intracellularly retained GLP-1R. B: MIN6 cells expressing GLP-1R-GFP and mCherry SUMO-1 transduced with shRNA Ubc-9 shows plasma membrane localization of GLP-1R. C: graph showing the ratio of mean fluorescence at the membrane vs. cytosol from 10 randomly selected cells. Partial knock down of Ubc-9 results in a 2.8-fold increase in plasma membrane localization. Error bars indicate means ± SD. Student's t-test: ***P < 0.0007; n = 10 cells from multiple dishes. Scale bar = 5 μm.