Table 1.

Primers for dopamine and angiotensin systems in the cultured proximal tubule epithelial cells

Primers	Forward	Reverse	Product
AADC	GTTTGTGCTACGCTTTGCTG	CGAAGACGGAGTGGTAGTTA	200 bp
COMT	GCCAGGCTTCTCACCATG	TCGTACTTCTTCTTCAGCT	143 bp
MAO-A	GCAACACAGTGGAGTGGCTA	GCCACAGAAGTGGAAAAACC	224 bp
MAO-B	GTGGTATGTGAAGCAGTGTGG	TCAGTGCCTGCAAAGAAAATC	723 bp
DAT	ATCATTGCCACATCCTCCAT	CATATGGCAAAAGGGGACTG	295 bp
D1	AACTGTATGGTGCCCTTCTGTGG	GCCCCGTTGTTGTTGATGCTTACA	230 bp
D2	CACTCCGCCACTTCTTGACATACA	AGCCCCATCCACAGCCTCCTCT	208 bp
D3	GTCCTGCCCTCTCCTCTTTGGTTT	CATTTGTCCCGTGGCATCTGA	310 bp
D4	TGCCCTCAACCCCATCATCTACAC	TCCCAACCCCCAGCCTTCATAA	202 bp
D5	GGGAGATCGCTGCTGCCTATGTC	ATTGGGGGTGAGTGGTGAGATTTT	221 bp
AT1a	GACCAACTCAACCCAGAAAAGC	CGAAGCGATCTTACATAGGTG	340 bp
AT1b	TGGGAATATTTGGAAACAGTTTGGT	CAGAGTATAGCTGGTGAGAATAAT	530 bp
AT2	TCCTGTTCTCTACTACATGATTTTTG	AGCCATAATACAAGCATTCACAC	454 bp
Mas	ACTGCCGGGCGGTCATCATC	GGTGGAGAAAAGCAAGGAGA	263 bp
AGT	CAAATGCACAGATCGGAGATGAC	CAGGTGCTCTTGTTGTGGTAAAGG	143 bp
ACE	TAACTCGAGTGCCGAGGTG	CCAGCAGGTGGCAGTCTT	340 bp
ACE2	CTTCAGCACTCTCAGCAGACA	CAACTTCCTCCTCACATAGGC	431 bp

AADC, aromatic l-amino acid decarboxylase; COMT, catechol-O-methyl-transferase; MAO, monoamine oxidase; DAT, dopamine transporter; AT_1a and AT_1b, angiotensin II receptors type 1a and 1b; AT₂, angiotensin II receptor type 2; Mas, Ang 1-7 receptor; AGT, angiotensinogen; ACE, angiotensin-converting enzyme.