Fig. 4.
Representative images of immunodetectable von Willebrand factor in en face aorta and analysis of fluorescence intensity. A: endothelial layer of en face aorta 3 h after IRI from normal control (top left), ITF 1697-treated control (top right), IRI (bottom left), and ITF 1697-treated IRI (bottom right) groups stained with anti- von Willebrand factor antibodies. Scale bar = 30 μm. B: graphs summarizing fluorescence intensity in each group; n = 6. *P < 0.05.