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. 2011 Dec 21;302(6):F730–F741. doi: 10.1152/ajprenal.00520.2011

Fig. 2.

Fig. 2.

HMGB1 translocation in HUVEC treated with UA. Quantified graphs and representative gels depict HMGB1 protein abundance in nuclear and cytoplasmic fractions after UA (50 μg/ml) treatment. HMGB1 decreased in nuclear fractions (A) while increasing in cytoplasmic fractions (B) after 15 and 60 min of UA treatment. Immunocytochemistry images (C) of HUVEC treated with UA for 15 and 60 min, fixed, and stained with fluorescent HMGB1 antibodies show an increase in cytoplasmic fluorescence 15 and 60 min after UA treatment, indicative of HMGB1 movement from nucleus to cytoplasm. Graphs depicting quantified fluorescence intensity of HMGB1 in nuclei (D) and cytoplasm (E) indicate that upon UA treatment HMGB1 translocates from the nucleus to the cytoplasm. *P < 0.05 vs. control. ∧P < 0.05 vs. 60; n = 5. Magnification ×100.