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. 2011 Dec 21;302(6):F730–F741. doi: 10.1152/ajprenal.00520.2011

Fig. 7.

Fig. 7.

UA- and HMGB1-mediated HMGB1 translocation is blocked by ethyl pyruvate. Similar to UA, HUVEC treatment with 50 ng/ml of rHMGB1 reduced nuclear (A) while increasing cytoplasmic (C) and medium (E) HMGB1, an effect that was blocked by 25 mM ethyl pyruvate (EP). Treatment with rHMGB1 (indicative of a positive feedback mechanism) increased acetylation of nuclear HMGB1 (B), and following translocation, acetylated HMGB1 subsequently increased in the cytoplasm (D) and medium (F), an effect also inhibited by EP. Representative gel lanes: lane 1, control; lane 2, UA; lane 3, UA+EP; lane 4, rHMGB1; and lane 5, rHMGB1+EP. *P < 0.05 vs. control. †P < 0.05 vs. EP; n = 8.