Fig. 8.

NF-κB activity in HUVEC is elevated by UA and rHMGB1, an effect inhibited by EP. NF-κB luciferase reporter assay was measured after HUVEC treatment with either UA (50 μg/ml) or rHMGB1 (50 ng/ml; with or without 25 mM EP) for 24 h. NF-κB activity increased upon UA and rHMGB1 treatment, an effect significantly attenuated by EP treatment or MyD88 small interfering siRNA. Transfection of control scrambled siRNA did not affect UA- or rHMGB1-mediated increase in NF-κB activity (data not shown). *P < 0.05 vs. control, EP, MyD88 siRNA. #P < 0.05 vs. control, EP; n = 6.