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. 2011 Dec 21;302(6):F730–F741. doi: 10.1152/ajprenal.00520.2011

Fig. 9.

Fig. 9.

Upregulation of angiopoietin-2 (ANG 2) by HMGB1 in HUVEC. HUVEC were treated with 3 different concentrations of rHMGB1: 10, 50, and 100 ng/ml. Treatment of HUVEC with 10 ng/ml of HMGB1 resulted in a significant increase in ANG 2 release into the medium within 15 min (A). However, a more robust increase was observed at 30 min with 50 and 100 ng/ml (A). ANG 2 mRNA expression in HUVEC was increased within 30 min of treatment with 50 and 100 ng/ml of rHMGB1 protein, but not with 10 ng/ml (B). ANG 2 mRNA was normalized to β-actin. ANG 2 protein in HUVEC increased within 30 min of treatment with all 3 concentrations of rHMGB1 (C), but treatment with 10 ng/ml of rHMGB1 caused an initial decline in HUVEC ANG 2 at 15 min, suggesting initial stimulated ANG 2 release from HUVEC at this concentration. *P < 0.05 vs. 0 min. #P < 0.05 vs. all time points. †P < 0.05 vs. 0 and 5 min. ±P < 0.05 vs. 15 min; n = 4.