Fig. 3.

Ligand induced degradation of EGFR is compromised in dynamin depleted cells. Serum starved control or DKO fibroblasts were pretreated with 10 μM of cycloheximide for 1 h followed by stimulation with 10 ng/mL (A) or 100 ng/mL (B) of EGF for indicated times. Equal amounts of cell lysates were subjected to immunobloting with anti-EGFR antibodies and as controls with anti-dynamin or anti-AKT antibodies. Arrow points to an EGFR degradation product that is detected in EGF stimulated WT cells and not in dynamin depleted cells. Similar results were obtained in three different experiments.