Fig. 5.

Similar stimulation of MAP kinase and AKT responses in dynamin depleted cells. Serum starved control or DKO fibroblasts were stimulated with 1.5 ng/mL (A), 5 ng/mL (B), or 100 ng/mL (C) of EGF. Cells were collected at the indicated time points and equal amounts of lysates were subjected to immunobloting with anti-AKT or anti-ERK antibodies. Membranes were reprobed with anti-pAKT or anti-pERK antibodies to monitor their enzymatic activities. EGFR and dynamin levels were monitored by inmunoblotting with anti-EGFR or anti-dynamin antibodies respectively. Similar results were obtained in three different experiments.