Fig. 2.

A conserved N-terminal region of FAAP20 is required for its interaction with FANCA. (A) Illustration of deletion mutants of FAAP20 used in this study. (B and D) 293T cells were transfected with plasmids encoding SFB-FANCA along with those encoding Myc-tagged wild-type or mutants of FAAP20. Precipitation was conducted using streptavidin beads and immunoblotting was performed using anti-Flag or anti-Myc antibodies as indicated. (C) Alignment of the N terminus of FAAP20 from different species. The conserved amino acids are shaded in black. Three mutants of FAAP20 with six alanine substitutions were generated (6A1, 6A2, and 6A3).