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. 2012 Mar 1;109(12):4639–4644. doi: 10.1073/pnas.1116269109

Fig. 1.

Fig. 1.

The 3′ UTR of sensorin is sufficient to target reporter mRNA into distal neurites. pNEX vectors encoding translational reporters were microinjected into isolated Aplysia SNs in culture (DIV 2). Neurons were fixed (DIV 4) and processed for FISH with dendra antisense riboprobes. (A) Cartoons of reporter constructs in pNEX3 expression vector. (B) Representative images of reporter (dendra2) mRNA FISH in isolated SNs. (B1–B6) Neurolucida tracing of each SN; (B1′–B6′) FISH (detected with dendra antisense riboprobes for B1′–B5′ and with sensorin antisense riboprobes for B6′). The FISH signal only extends to distal neurites when the 3′ UTR of sensorin is present; distal localization is enhanced by 5′ UTR. (Scale bar, 100 μm.) (C) Quantification of the distribution of reporter mRNA within sensory neurites. Neurites were linearized and divided into proximal, middle, and distal segments. The percentage of total FISH signal in distal segments is shown (see also Fig. S1). ***P < 0.0001, Kruskal-Wallis one-way analysis of variance followed by Dunnett's multiple comparison test.