Fig. 2.
OAMB is not required for sperm entry into storage but is required for the depletion of sperm from the seminal receptacle and spermathecae. (A) Distribution of the uterine conformational stages at 35 min after the start of mating in oamb286 mutant females compared with their sibling controls (NOAMB = 36, NTM6 = 36). Total sperm stored in the SSOs (B), in the seminal receptacle (C), and in the spermathecae (D) in oamb286 mutant females compared with their sibling controls. The difference between female genotypes in the depletion of stored sperm over time was analyzed using two-factor ANOVA. The significance of the genotype factor, indicating differences in sperm depletion between oamb286 mutant and control females, is reported in the figure as follows: + = 0.005 < P < 0.05 or ++ = P < 0.005 (an additional explanation of the statistical analysis is provided in Materials and Methods). Sp, spermathecae; SR, seminal receptacle. Sample sizes for sperm counts range from n = 11–15 (Table S1).