Fig. 1.

SapC, but not SapB, makes stable contacts with lipid bilayer. (A) SapC was incubated with liposomes for 60 min in sodium citrate buffer, pH 5.0. Liposomes and SapC samples were transferred to a sucrose gradient buffered to pH 5 or pH 7 where indicated, and centrifuged at 200,000 × g. Gradient fractions were collected, separated by SDS/PAGE, and visualized by silver staining. (B) SapB was incubated with liposomes, transferred to a sucrose gradient and visualized as described in A. “Top” and “bottom” indicate low and high-density sucrose gradient fractions, respectively. Triangle (Bottom) indicates increasing sucrose density.