Fig. 3.
Liposome bilayer enhances CD1c-Fc–SapC interactions. (A) SapC and Fc fusion proteins were coupled to sulfhydryl-reactive and sulfhydryl-containing cross-linkers, respectively, and incubated with or without liposomes at pH 5.0 and pH 7.0. Samples were separated by nonreducing PAGE and immunoblotted with anti-SapC antibodies. (B) Total CD1c-Fc and MHC-I-Fc fusion proteins were separated by SDS/PAGE and visualized by silver stain.