Fig. 1.
The spectra and photochemical kinetics of mGeos. (A) The excitation (measured at the emission maximum) and emission (measured at the excitation maximum) spectra of photoactivatable mGeos and mEos2. (B) The absorbance spectra of mGeos and mEos2 in their bright states. (C) Single switching kinetics for His62 mutant mGeos, Dronpa, and PDM1-4. (D) Single switching kinetics for His62 and F173S double mutant mGeos and rsFastLime. The blue solid line is mGeos-C, used as a control. The laser intensity in (C) is 15-fold higher than in (D).