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. 2012 Feb 24;109(12):E690-E697. doi: 10.1073/pnas.1115485109

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Fig. 5. — Dynamic force spectroscopy of the SpyTag:SpyCatcher interaction. (A) Cartoon of the constructs for testing the SpyTag:SpyCatcher interaction by AFM. (B) Representative force extension curve for Cys-I27₂-SpyCatcher forming a strong interaction with SpyTag-MBP-Cys, showing regions corresponding to PEG/agarose stretching, unfolding of two I27 domains (enlarged in the inset), and covalent bond breakage. (C) Histogram of different bond breakage forces for Cys-I27₂-SpyCatcher interactions with SpyTag-MBP-Cys. (D) Table of statistics of breakage forces. SpyTag:SpyCatcher was compared with the control SpyTag:SpyCatcher EQ. SpyTag was also tested against a cantilever only coated with PEG (SpyTag:PEG), or SpyTag was preblocked with free SpyCatcher before testing with a SpyCatcher-coated cantilever.