FIG. 1.

Fluorescent imaging outputs. (a) Typical fluorescent image of inguinal-to-axillary collecting lymph vessel (flank lymphatics also appear) with regions of interest (ROIs) selected (yellow boxes). (b) Fluorescence intensity in arbitrary units plotted against seconds. ROI 1 values are shown in blue, and ROI 2 values are shown in red. Each peak represents one pulse. The difference between ROI 1 and ROI 2 time peaks is used to calculate lymph propulsive velocity. A color version of this figure is available in the online article at www.liebertpub.com/lrb.