Lymphatic function is not affected by indocyanine green (ICG) concentration. (a) Lymphatic propulsive frequencies (pulses/minute) and (b) lymph propulsive velocities (centimeters/second) observed in inguinal-to-axillary postnodal efferent transport lymphatic vessels using 0.32, 0.645, and 1.3 mM ICG as fluorescent dye. Light gray bars denote left side values, while dark gray bars denote right side values. N=12 mice per group for 0.32, 0.645, and 1.3 mM ICG. No statistically significant differences between/among groups were found by linear mixed effects model (velocities) or ANOVA (frequencies).