Fig. 3.
Rosiglitazone abrogated ethanol-stimulated adipose lipolysis and the relative gene expression. Mice were fed ethanol liquid diet for 8 wk, and rosiglitazone was supplemented in the liquid diet at 10 mg·kg body wt−1·day−1 for the last 3 wk. A: WAT lipolysis. Lipolysis was estimated by measuring free fatty acid (FFA) release from WAT. Epididymal WAT tissue explants were cultured for 2 h and fatty acids released to the media were measured with a FFA quantification kit. B: qRT-PCR analysis of gene expression related to lipolysis. Data are expressed as means ± SD; n = 6–8 in A; n = 4 in B. Means without a common letter differ at P < 0.01.