Fig. 6.

Berberine decreases TNF (A) and KC (B) production in colonic epithelial cells from DSS-treated mice. Mice were treated as shown in Fig. 2. Colon tissues were collected for isolation of colonic epithelial cells. mRNA was isolated from the colonic epithelial cells for real-time PCR analysis of the indicated cytokine mRNA expression levels. The cytokine mRNA expression level in water group was set as 100%, and mRNA expression levels in treated mice were compared with the water group. *P < 0.05 compared with water group and DSS+Ber group; n = at least 5 mice in each group.