Fig. 1.
The ischemia-reperfusion (I/R) protocol. Time 0 is the start of cardiopulmonary bypass. Baseline hemodynamic and plasma data were performed before time 0. The aortic cross clamp was placed at time 20 min and released at time 80 min. Cardiopulmonary bypass weaning started at time 90 min and was completed by time 110 min. Intracoronary [2-13C]pyruvate infusion was given to groups I/R and I/R-Tr for 40 min starting 10 min after release of aortic cross clamp. In the I/R-Tr group only, 0.6 μg·kG−1·dose−1 iv triiodothyronine (T3) was given during reperfusion at the times denoted by T3 in the figure. Final hemodynamic measurements were performed near pyruvate infusion completion. Left ventricular tissue was collected at the end of the infusion. Inset: control protocol. Intracoronary [2-13C]pyruvate was infused for 40 min starting at time 0. Left ventricular tissue was collected at the end of the infusion.