Effects of N-hydroxy-N-(4-butyl-2-methylphenyl)formamidine (HET0016), 20-hydroxyeicosa-6(Z),15(Z)-dienoic acid (6,15-20-HEDE), and 20-hydroxyeicosa-5(Z),14(Z)-dienoic acid (5,14-20-HEDE) on oxygen-glucose deprivation (OGD)- and reoxygenation-induced lactate dehydrogenase (LDH) release. HET0016 (10 μM), 6,15-20-HEDE (10 μM), 5,14-20-HEDE (10 and 30 μM), or vehicle (ethanol) were added to the media of organotypic hippocampal slices 30 min before the slices were subjected to OGD and reoxygenation and were present throughout the experiment. LDH release was measured at the end of the 2-h reoxygenation period in the incubation media. Results are mean values ± SE of at least 5 experiments/group. *P < 0.05 vs. the OGD group pretreated with vehicle.