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. 2012 Jan 13;302(6):H1285–H1293. doi: 10.1152/ajpheart.00340.2011

Fig. 3.

Fig. 3.

Effect of HET0016 on 20-15-hydroxyeicosatetraenoic acid (20-HETE) levels in hippocampal slice cultures subjected to OGD followed by reoxygenation. Hippocampal slices were treated with HET0016 (10 μM) or vehicle for 30 min before OGD and throughout the experiment. Free levels of 20-HETE in hippocampal slices were determined by liquid chromotography-tandem mass spectroscopy (LC-MS/MS) 15 min after reoxygenation. A: representative LC-MS/MS chromatogram. TXB2, thromboxane B2. B: bar graph showing the levels of 20-HETE under control conditions, after OGD followed by reoxygenation, and after OGD in slices pretreated with HET0016 (10 μM). Values are means ± SE of 4 experiments/group. *P < 0.05 vs. the control group; †P < 0.05 vs. the OGD group.