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. 2012 Jan 13;302(6):H1285–H1293. doi: 10.1152/ajpheart.00340.2011

Fig. 5.

Fig. 5.

Effect of HET0016 and 5,14-20-HEDE on superoxide production by hippocampal slices subjected to OGD and reoxygenation. Hippocampal slices were pretreated with vehicle, HET0016 (10 μM), and 5,14-20-HEDE (10 and 30 μM) 30 min before OGD and throughout the experiment. At the beginning of the reoxygenation period, slices were placed in fresh HBSS containing 10 μM dihydroethidium (DHE), and DHE fluorescence was measured using a fluorescence microtiter plate reader. Manganese III tetrakis (1-methyl-4-pyridyl)porphyrin pentachloride (MnTMPyP; 50 μM) was added to the slices exposed to OGD at the beginning of the reoxygenation period. A: quantitative comparison of DHE fluorescence intensity measured during the different experimental conditions as indicated in the graph. Values are means ± SE from 7 experiments/group. *P < 0.05 vs. the OGD group. B: representative images showing increased DHE fluorescence after exposure to OGD and treatment with the 20-HETE analog 5,14-20-HEDE or decreased levels after treatment with HET0016 or MnTMPyP.