Fig. 6.

Effects of HET0016 and 5,14-20-HEDE on caspase-3 activation in hippocampal slice cultures subjected to OGD followed by reoxygenation. Hippocampal slices were pretreated with vehicle, HET0016 (10 μM), or 5,14-20-HEDE (10 and 30 μM) 30 min before OGD and throughout the experiment. Slices were subjected to 90 min of OGD followed by 2 h of reoxygenation. A: comparison of caspase-3 activity measured using a fluorometric assay in the various groups. Values are means ± SE from 5 experiments/group. *P < 0.05 vs. the OGD group. B: representative Western blot showing caspase-3 protein levels in hippocampal slices under control conditions, after exposure to OGD and reoxygenation, or after pretreatment with the 20-HETE synthesis inhibitor HET0016 (10 μM) or the 20-HETE mimetic 5,14-20-HEDE (30 μM). β-Actin was used as a protein loading control.