Fig. 2.

RUPP and CoPP treatment changes placental phosphorylation of STATs in rats. Pregnant rats were subjected to RUPP and then treated with CoPP or vehicle as described in materials and methods. A group of NP rats were also treated with either CoPP or vehicle. On gestational day 19, placentas were excised and processed to obtain protein lysates. All groups included three placentas from different animals. A: tyrosine phosphorylation of STAT3 was determined by Western blot analysis and compared with the unphosphorylated STAT3. B: densitometry of data in A. Ratio of pTyrSTAT3/STAT3 was calculated. ^aP < 0.05 compared with NP, ^bP < 0.05 compared with RUPP. C: tyrosine phosphorylation of STAT1 was determined by Western blot analysis and compared with the unphosphorylated STAT1. D: densitometry of data in C. Ratio of pTyrSTAT1/STAT1 was calculated. ^aP < 0.05 compared with NP; ^bP < 0.05 compared with RUPP.