Fig. 3.

RUPP and CoPP treatment changes placental phosphorylation of shc proteins in rats. Pregnant rats were subjected to RUPP and then treated with CoPP or vehicle, as described in materials and methods. A group of NP rats were also treated with either CoPP or vehicle. On gestational day 19, placentas were excised and processed to obtain protein lysates. All groups included three placentas from different animals. A: tyrosine phosphorylation of shc proteins was determined by Western blot analysis and compared with the unphosphorylated shc. B: densitometry of data in A. Ratio of pTyrp52shc/p52shc was calculated. ^aP < 0.05 compared with NP.