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RT-PCR conditions for the different primers
| Gene | Cycle Profile |
|---|---|
| eNOS | 95°C/30 s; 58°C/30 s; 72°C/45 s for 40 cycles |
| COX1 | 95°C/30 s; 58°C/30 s; 72°C/1 min for 35 cycles |
| COX2 | 95°C/30 s; 58°C/30 s; 72°C/1 min for 35 cycles |
| GAPDH | 95°C/30 s; 58°C/30 s; 72°C/1 min for 35 cycles |
