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. Author manuscript; available in PMC: 2012 Oct 21.
Published in final edited form as: ACS Chem Biol. 2011 Aug 22;6(10):1029–1035. doi: 10.1021/cb2001395

Figure 3. Selective reduction of mesoridazine affects cell viability.

Figure 3

(A) Cell viability analysis of HEK 293 cells expressing GFP (control), GFP-fused mMsrAΔS, or GFP-fused mMsrB2ΔS following treatment of cells with mesoridazine (3, 6, 12.5, 25, 50, 100 μM) for 24 h. Constructs were designed to express proteins in the cytosol (where indicated by removing the signal peptide marked as ΔS). (B) Cell viability analysis of HEK 293 cells transfected with GFP (control), GFP-fused mMsrAΔS, or GFP-fused mMsrB2ΔS following treatment with thioridazine (3, 6, 12.5, 25, 50, 100 μM) for 24 h. Constructs were designed to express proteins in the cytosol (ΔS indicates that the signal peptide was removed). All measurements were repeated 6 times independently, and the data analyzed with a Student's t-test (**: p<0.01 and *: p<0.05). (C) An overall scheme of stereospecific reduction of methylsulfinyl-containing drugs.