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. 2012 Mar 23;7(3):e33853. doi: 10.1371/journal.pone.0033853

Figure 4. CASP8 gene promoter CGI methylation and TNF-α expression in mouse livers.

Figure 4

Mice were treated with Cd at 20 nmol/kg for 4 weeks and then were sacrificed at the 56th week after Cd exposure. The Cd-treated and the age-matched mice were given with or without the methylation inhibitor 5-aza for 6 weeks (i.e.: 4 weeks during and 2 weeks after 4-week Cd-treatment). Liver tissues of these four groups of mice were collected for the analysis of CGI methylation status for CASP8 gene promoter with EpiTect Methyl qPCR assay (A), c-CASP8 expression by Western blotting (B), and TNF-α expression by Western blot (C). Data was presented as mean ± SD (n = 10). * p<0.05 vs control; # p<0.05 vs Cd group.