Figure 5. Linear relationship between area diameter (D_(a)) and apparent volume diameter (D_(v)) of normal RGCs.

D_(a) was estimated by image-analysis software. Apparent (D_(v)) was obtained by substituting its FSC value in the calibration curve of the standard microspheres (y = 0.027x+2.16 x = FSC value; y = diameter). (D_{(a, 5)}, D_{(v, 5)}), (D_{(a, 20)}, D_{(v, 20)}), (D_{(a, 40)}, D_{(v, 40)}), (D_{(a, 60)}, D_{(v, 60)}), (D_{(a, 80)}, D_{(v, 80)}), and (D_{(a, 95)}, D_{(v, 95)}) were plotted to obtain the equation: y = 1.61x−1.94. D_{(v, Q)} and D_{(a, Q)} represent the volume diameter and area diameter, respectively at the cumulative cell size percentile of Q in total RGCs.