Skip to main content
. 2012 Mar 23;7(3):e33965. doi: 10.1371/journal.pone.0033965

Figure 3. The expression of adiponectin, AdipoR1 and AdipoR2 genes in cultured human mesangial cells (HMCs).

Figure 3

(A) The cDNA from human adipocytes was utilized as a positive control for adiponectin and the cDNA from human hepatocytes was used as a control for AdipoR1 and AdipoR2. RT-PCR for adiponectin in HMCs after stimulation with either native or deSial/deGal IgA1 afforded cDNA bands of the same size (256 bp) as that amplified from human adipocyte cDNA. RT-PCR for AdipoR1 in HMCs afforded cDNA bands of the same size (70 bp) as that amplified from human hepatocytes. The HMCs did not express AdipoR2 mRNA. The expression of the GAPDH gene was used as an internal standard (350 bp). (B) The densitometric analysis of the expression of each cDNA.