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NC-specific IgM (A), IgG1 (B), IgG2b (C), IgG2c (D), IgG3 (E), IgA (F), IgG (G), and Ig (H) titers were determined by ELISA on plates coated with disrupted viral particles. Titers are shown as the reciprocal of the highest serum dilution scored as positive relative to naïve control serum. The mean+SE is shown for 4–8 individual mice per group. * P<0.05, ** P<0.01.
